anti caveolin 1 Search Results


rabbit anti caveolin 1 antibody  (Miltenyi Biotec)
92
Miltenyi Biotec rabbit anti caveolin 1 antibody
Rabbit Anti Caveolin 1 Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti caveolin 1  (Boster Bio)
90
Boster Bio anti caveolin 1
Anti Caveolin 1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cav 1 boster biological technology  (Boster Bio)
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Boster Bio cav 1 boster biological technology
Cav 1 Boster Biological Technology, supplied by Boster Bio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mab anti cav 1  (Bio-Rad)
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Bio-Rad mab anti cav 1
Mab Anti Cav 1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cav1 2 signal  (Bio-Rad)
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Bio-Rad cav1 2 signal
Cav1 2 Signal, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cav 1 antibody  (Boster Bio)
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Boster Bio cav 1 antibody
Cav 1 Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti-caveolin-1  (Becton Dickinson)
90
Becton Dickinson mouse anti-caveolin-1
Colocalization of AQP2 and <t>caveolin-1.</t> Confluent MDCK-hAQP2 cells grown on permeable supports without any treatment as a control ( a–c ), treated with forskolin for 30 min ( d–f ), or treated with forskolin for 30 min, washed, and then incubated without forskolin for 30 min ( g–i ) were fixed. Cells were sectioned perpendicular to the supports with a cryostat and double-immunofluorescently labeled for AQP2 (red) and caveolin-1 (green). Nuclei were stained with DAPI (blue). Merged confocal images are shown on the left. In the control, AQP2 is seen in the subapical cytoplasm and seems not to colocalize with caveolin-1, which is present on the apical membrane. AQP2 is largely colocalized with caveolin-1 on the apical membrane upon forskolin treatment. Some AQP2 internalized after washout is colocalized with caveolin-1. Bar=10 µm.
Mouse Anti Caveolin 1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-caveolin1 antibody  (Cloud-Clone corp)
90
Cloud-Clone corp anti-caveolin1 antibody
Colocalization of AQP2 and <t>caveolin-1.</t> Confluent MDCK-hAQP2 cells grown on permeable supports without any treatment as a control ( a–c ), treated with forskolin for 30 min ( d–f ), or treated with forskolin for 30 min, washed, and then incubated without forskolin for 30 min ( g–i ) were fixed. Cells were sectioned perpendicular to the supports with a cryostat and double-immunofluorescently labeled for AQP2 (red) and caveolin-1 (green). Nuclei were stained with DAPI (blue). Merged confocal images are shown on the left. In the control, AQP2 is seen in the subapical cytoplasm and seems not to colocalize with caveolin-1, which is present on the apical membrane. AQP2 is largely colocalized with caveolin-1 on the apical membrane upon forskolin treatment. Some AQP2 internalized after washout is colocalized with caveolin-1. Bar=10 µm.
Anti Caveolin1 Antibody, supplied by Cloud-Clone corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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the rabbit anti-caveolin 1 pab  (Becton Dickinson)
90
Becton Dickinson the rabbit anti-caveolin 1 pab
Colocalization of AQP2 and <t>caveolin-1.</t> Confluent MDCK-hAQP2 cells grown on permeable supports without any treatment as a control ( a–c ), treated with forskolin for 30 min ( d–f ), or treated with forskolin for 30 min, washed, and then incubated without forskolin for 30 min ( g–i ) were fixed. Cells were sectioned perpendicular to the supports with a cryostat and double-immunofluorescently labeled for AQP2 (red) and caveolin-1 (green). Nuclei were stained with DAPI (blue). Merged confocal images are shown on the left. In the control, AQP2 is seen in the subapical cytoplasm and seems not to colocalize with caveolin-1, which is present on the apical membrane. AQP2 is largely colocalized with caveolin-1 on the apical membrane upon forskolin treatment. Some AQP2 internalized after washout is colocalized with caveolin-1. Bar=10 µm.
The Rabbit Anti Caveolin 1 Pab, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-caveolin1  (System Biosciences Inc)
90
System Biosciences Inc anti-caveolin1
Colocalization of AQP2 and <t>caveolin-1.</t> Confluent MDCK-hAQP2 cells grown on permeable supports without any treatment as a control ( a–c ), treated with forskolin for 30 min ( d–f ), or treated with forskolin for 30 min, washed, and then incubated without forskolin for 30 min ( g–i ) were fixed. Cells were sectioned perpendicular to the supports with a cryostat and double-immunofluorescently labeled for AQP2 (red) and caveolin-1 (green). Nuclei were stained with DAPI (blue). Merged confocal images are shown on the left. In the control, AQP2 is seen in the subapical cytoplasm and seems not to colocalize with caveolin-1, which is present on the apical membrane. AQP2 is largely colocalized with caveolin-1 on the apical membrane upon forskolin treatment. Some AQP2 internalized after washout is colocalized with caveolin-1. Bar=10 µm.
Anti Caveolin1, supplied by System Biosciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-caveolin-1 mab  (BioAmerica Inc)
90
BioAmerica Inc anti-caveolin-1 mab
Colocalization of AQP2 and <t>caveolin-1.</t> Confluent MDCK-hAQP2 cells grown on permeable supports without any treatment as a control ( a–c ), treated with forskolin for 30 min ( d–f ), or treated with forskolin for 30 min, washed, and then incubated without forskolin for 30 min ( g–i ) were fixed. Cells were sectioned perpendicular to the supports with a cryostat and double-immunofluorescently labeled for AQP2 (red) and caveolin-1 (green). Nuclei were stained with DAPI (blue). Merged confocal images are shown on the left. In the control, AQP2 is seen in the subapical cytoplasm and seems not to colocalize with caveolin-1, which is present on the apical membrane. AQP2 is largely colocalized with caveolin-1 on the apical membrane upon forskolin treatment. Some AQP2 internalized after washout is colocalized with caveolin-1. Bar=10 µm.
Anti Caveolin 1 Mab, supplied by BioAmerica Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-caveolin-1 mab/product/BioAmerica Inc
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rabbit anti-caveolin 1 antibodies  (Interchim Chemicals)
90
Interchim Chemicals rabbit anti-caveolin 1 antibodies
Colocalization of AQP2 and <t>caveolin-1.</t> Confluent MDCK-hAQP2 cells grown on permeable supports without any treatment as a control ( a–c ), treated with forskolin for 30 min ( d–f ), or treated with forskolin for 30 min, washed, and then incubated without forskolin for 30 min ( g–i ) were fixed. Cells were sectioned perpendicular to the supports with a cryostat and double-immunofluorescently labeled for AQP2 (red) and caveolin-1 (green). Nuclei were stained with DAPI (blue). Merged confocal images are shown on the left. In the control, AQP2 is seen in the subapical cytoplasm and seems not to colocalize with caveolin-1, which is present on the apical membrane. AQP2 is largely colocalized with caveolin-1 on the apical membrane upon forskolin treatment. Some AQP2 internalized after washout is colocalized with caveolin-1. Bar=10 µm.
Rabbit Anti Caveolin 1 Antibodies, supplied by Interchim Chemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Colocalization of AQP2 and caveolin-1. Confluent MDCK-hAQP2 cells grown on permeable supports without any treatment as a control ( a–c ), treated with forskolin for 30 min ( d–f ), or treated with forskolin for 30 min, washed, and then incubated without forskolin for 30 min ( g–i ) were fixed. Cells were sectioned perpendicular to the supports with a cryostat and double-immunofluorescently labeled for AQP2 (red) and caveolin-1 (green). Nuclei were stained with DAPI (blue). Merged confocal images are shown on the left. In the control, AQP2 is seen in the subapical cytoplasm and seems not to colocalize with caveolin-1, which is present on the apical membrane. AQP2 is largely colocalized with caveolin-1 on the apical membrane upon forskolin treatment. Some AQP2 internalized after washout is colocalized with caveolin-1. Bar=10 µm.

Journal: Acta Histochemica et Cytochemica

Article Title: Close Association of Aquaporin-2 Internalization with Caveolin-1

doi: 10.1267/ahc.12003

Figure Lengend Snippet: Colocalization of AQP2 and caveolin-1. Confluent MDCK-hAQP2 cells grown on permeable supports without any treatment as a control ( a–c ), treated with forskolin for 30 min ( d–f ), or treated with forskolin for 30 min, washed, and then incubated without forskolin for 30 min ( g–i ) were fixed. Cells were sectioned perpendicular to the supports with a cryostat and double-immunofluorescently labeled for AQP2 (red) and caveolin-1 (green). Nuclei were stained with DAPI (blue). Merged confocal images are shown on the left. In the control, AQP2 is seen in the subapical cytoplasm and seems not to colocalize with caveolin-1, which is present on the apical membrane. AQP2 is largely colocalized with caveolin-1 on the apical membrane upon forskolin treatment. Some AQP2 internalized after washout is colocalized with caveolin-1. Bar=10 µm.

Article Snippet: Primary antibodies were as follows: rabbit anti-AQP2 [ , ], guinea pig anti-AQP2 [ , ], goat anti-AQP2 (sc-9882; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-Rab11a (Zymed, San Francisco, CA), mouse anti-EEA1 (clone 14; BD Biosciences, San Jose, CA), goat anti-EEA1 (sc-6414, Santa Cruz Biotechnology), mouse anti-caveolin-1 (clone 2234, BD Biosciences), rabbit anti-caveolin-1 (sc-894, Santa Cruz Biotechnology), mouse anti-phosphorylated caveolin-1 (clone 56, BD Biosciences), rabbit anti-cathepsin D [ ], and mouse anti-gp135 [ ].

Techniques: Incubation, Labeling, Staining

AQP2 is recovered in Triton X-100-insoluble fraction. Samples of Triton X-100-soluble (S) and -insoluble (I) fractions from MDCK-hAQP2 cell lysates with (+) or without (–) forskolin treatment for 30 min were subjected to SDS-PAGE and immunoblotting using anti-AQP2 and anti-caveolin-1 (Cav1) antibodies.

Journal: Acta Histochemica et Cytochemica

Article Title: Close Association of Aquaporin-2 Internalization with Caveolin-1

doi: 10.1267/ahc.12003

Figure Lengend Snippet: AQP2 is recovered in Triton X-100-insoluble fraction. Samples of Triton X-100-soluble (S) and -insoluble (I) fractions from MDCK-hAQP2 cell lysates with (+) or without (–) forskolin treatment for 30 min were subjected to SDS-PAGE and immunoblotting using anti-AQP2 and anti-caveolin-1 (Cav1) antibodies.

Article Snippet: Primary antibodies were as follows: rabbit anti-AQP2 [ , ], guinea pig anti-AQP2 [ , ], goat anti-AQP2 (sc-9882; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-Rab11a (Zymed, San Francisco, CA), mouse anti-EEA1 (clone 14; BD Biosciences, San Jose, CA), goat anti-EEA1 (sc-6414, Santa Cruz Biotechnology), mouse anti-caveolin-1 (clone 2234, BD Biosciences), rabbit anti-caveolin-1 (sc-894, Santa Cruz Biotechnology), mouse anti-phosphorylated caveolin-1 (clone 56, BD Biosciences), rabbit anti-cathepsin D [ ], and mouse anti-gp135 [ ].

Techniques: SDS Page, Western Blot

AQP2 co-immunoprecipitates with caveolin-1. a : In immunoprecipitates with anti-AQP2, caveolin-1 is detected as a dense band in forskolin-treated cells (F) and a faint band in control cells (C) at 22–24 kDa (arrow) with anti-caveolin-1 (Cav1). Dense bands both around 20 kDa (arrowhead) and 40 kDa (double-arrowhead) seem to be non-specific because these bands are also detected in the lane in which rabbit IgG was electrophoresed (RbIgG). b : Immunoprecipitates with anti-AQP2 from each cell lysate described as follows were subjected to immunoblotting with anti-caveolin-1 (Cav1). –: control cells, +: cells treated with forskolin for 30 min, ±: cells treated with forskolin for 30 min, washed, and then incubated without forskolin for 30 min.

Journal: Acta Histochemica et Cytochemica

Article Title: Close Association of Aquaporin-2 Internalization with Caveolin-1

doi: 10.1267/ahc.12003

Figure Lengend Snippet: AQP2 co-immunoprecipitates with caveolin-1. a : In immunoprecipitates with anti-AQP2, caveolin-1 is detected as a dense band in forskolin-treated cells (F) and a faint band in control cells (C) at 22–24 kDa (arrow) with anti-caveolin-1 (Cav1). Dense bands both around 20 kDa (arrowhead) and 40 kDa (double-arrowhead) seem to be non-specific because these bands are also detected in the lane in which rabbit IgG was electrophoresed (RbIgG). b : Immunoprecipitates with anti-AQP2 from each cell lysate described as follows were subjected to immunoblotting with anti-caveolin-1 (Cav1). –: control cells, +: cells treated with forskolin for 30 min, ±: cells treated with forskolin for 30 min, washed, and then incubated without forskolin for 30 min.

Article Snippet: Primary antibodies were as follows: rabbit anti-AQP2 [ , ], guinea pig anti-AQP2 [ , ], goat anti-AQP2 (sc-9882; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-Rab11a (Zymed, San Francisco, CA), mouse anti-EEA1 (clone 14; BD Biosciences, San Jose, CA), goat anti-EEA1 (sc-6414, Santa Cruz Biotechnology), mouse anti-caveolin-1 (clone 2234, BD Biosciences), rabbit anti-caveolin-1 (sc-894, Santa Cruz Biotechnology), mouse anti-phosphorylated caveolin-1 (clone 56, BD Biosciences), rabbit anti-cathepsin D [ ], and mouse anti-gp135 [ ].

Techniques: Western Blot, Incubation

Internalized caveolin-1 is phosphorylated. Samples of Triton X-100-insoluble fractions from MDCK-hAQP2 cell lysates were subjected to SDS-PAGE and immunoblotting using anti-phosphorylated caveolin-1 antibody. A strong band is detected in cells treated with forskolin and subsequently washed and incubated without forskolin for 30 min (±). Faint bands are detected in cells both treated (+) and untreated (–) with forskolin.

Journal: Acta Histochemica et Cytochemica

Article Title: Close Association of Aquaporin-2 Internalization with Caveolin-1

doi: 10.1267/ahc.12003

Figure Lengend Snippet: Internalized caveolin-1 is phosphorylated. Samples of Triton X-100-insoluble fractions from MDCK-hAQP2 cell lysates were subjected to SDS-PAGE and immunoblotting using anti-phosphorylated caveolin-1 antibody. A strong band is detected in cells treated with forskolin and subsequently washed and incubated without forskolin for 30 min (±). Faint bands are detected in cells both treated (+) and untreated (–) with forskolin.

Article Snippet: Primary antibodies were as follows: rabbit anti-AQP2 [ , ], guinea pig anti-AQP2 [ , ], goat anti-AQP2 (sc-9882; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-Rab11a (Zymed, San Francisco, CA), mouse anti-EEA1 (clone 14; BD Biosciences, San Jose, CA), goat anti-EEA1 (sc-6414, Santa Cruz Biotechnology), mouse anti-caveolin-1 (clone 2234, BD Biosciences), rabbit anti-caveolin-1 (sc-894, Santa Cruz Biotechnology), mouse anti-phosphorylated caveolin-1 (clone 56, BD Biosciences), rabbit anti-cathepsin D [ ], and mouse anti-gp135 [ ].

Techniques: SDS Page, Western Blot, Incubation

AQP2 is internalized to the same compartment with caveolin-1 and flotillin-2. ( a–f ) MDCK-hAQP2 cells were seeded on coverslips and subjected to forskolin treatment for 30 min ( a, d ) and subsequent washing and incubation without forskolin for 30 min ( b, e ) or for 2 hr ( c, f ). Double-immunofluorescence labeling for AQP2 and EEA1 ( a–c ) or for AQP2 and caveolin-1 ( d–f ) was carried out and their localization was observed with a laser confocal microscope. Projection images of 4 consecutive confocal images (0.4 µm intervals) are shown. AQP2 is shown in red. EEA1 and caveolin-1 are shown in green. An enlarged view of the rectangle area is shown in the inset ( f ). Both AQP2 and caveolin-1 are internalized in the EEA1-positive compartment 30 min after washing and then differentially localized at 2 hr. g, h : MDCK-hAQP2 cells transiently transfected with EGFP-flotillin-2 were treated with forskolin for 30 min ( g ) and then washed and incubated without forskolin for 30 min ( h ). Localization of AQP2 and flotillin-2 was observed with a laser confocal microscope. Projection images of 4 consecutive confocal images (0.4 µm intervals) are shown. AQP2 is shown in red. EGFP fluorescence is shown in green. Both AQP2 and EGFP-flotillin-2 are internalized in the same compartment. Bars=10 µm (5 µm in inset).

Journal: Acta Histochemica et Cytochemica

Article Title: Close Association of Aquaporin-2 Internalization with Caveolin-1

doi: 10.1267/ahc.12003

Figure Lengend Snippet: AQP2 is internalized to the same compartment with caveolin-1 and flotillin-2. ( a–f ) MDCK-hAQP2 cells were seeded on coverslips and subjected to forskolin treatment for 30 min ( a, d ) and subsequent washing and incubation without forskolin for 30 min ( b, e ) or for 2 hr ( c, f ). Double-immunofluorescence labeling for AQP2 and EEA1 ( a–c ) or for AQP2 and caveolin-1 ( d–f ) was carried out and their localization was observed with a laser confocal microscope. Projection images of 4 consecutive confocal images (0.4 µm intervals) are shown. AQP2 is shown in red. EEA1 and caveolin-1 are shown in green. An enlarged view of the rectangle area is shown in the inset ( f ). Both AQP2 and caveolin-1 are internalized in the EEA1-positive compartment 30 min after washing and then differentially localized at 2 hr. g, h : MDCK-hAQP2 cells transiently transfected with EGFP-flotillin-2 were treated with forskolin for 30 min ( g ) and then washed and incubated without forskolin for 30 min ( h ). Localization of AQP2 and flotillin-2 was observed with a laser confocal microscope. Projection images of 4 consecutive confocal images (0.4 µm intervals) are shown. AQP2 is shown in red. EGFP fluorescence is shown in green. Both AQP2 and EGFP-flotillin-2 are internalized in the same compartment. Bars=10 µm (5 µm in inset).

Article Snippet: Primary antibodies were as follows: rabbit anti-AQP2 [ , ], guinea pig anti-AQP2 [ , ], goat anti-AQP2 (sc-9882; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-Rab11a (Zymed, San Francisco, CA), mouse anti-EEA1 (clone 14; BD Biosciences, San Jose, CA), goat anti-EEA1 (sc-6414, Santa Cruz Biotechnology), mouse anti-caveolin-1 (clone 2234, BD Biosciences), rabbit anti-caveolin-1 (sc-894, Santa Cruz Biotechnology), mouse anti-phosphorylated caveolin-1 (clone 56, BD Biosciences), rabbit anti-cathepsin D [ ], and mouse anti-gp135 [ ].

Techniques: Incubation, Immunofluorescence, Labeling, Microscopy, Transfection, Fluorescence

Ultrastructural localization of AQP2 and caveolin-1. MDCK-hAQP2 cells were treated with forskolin for 30 min ( a ), and then washed and incubated without forskolin for 30 min ( b ). Ultrathin cryosections were double-labeled for AQP2 (12 nm colloidal gold) and caveolin-1 (6 nm colloidal gold). In forskolin-treated cells ( a ), some AQP2 is colocalized with caveolin-1 at caveolar structures in the apical membrane domain (arrows). AQP2 on the microvillous membrane is not colocalized with caveolin-1. When cells were washed and incubated without forskolin for 30 min ( b ), AQP2 was colocalized with caveolin-1 in the intracellular vesicles (arrows). Bars=100 nm.

Journal: Acta Histochemica et Cytochemica

Article Title: Close Association of Aquaporin-2 Internalization with Caveolin-1

doi: 10.1267/ahc.12003

Figure Lengend Snippet: Ultrastructural localization of AQP2 and caveolin-1. MDCK-hAQP2 cells were treated with forskolin for 30 min ( a ), and then washed and incubated without forskolin for 30 min ( b ). Ultrathin cryosections were double-labeled for AQP2 (12 nm colloidal gold) and caveolin-1 (6 nm colloidal gold). In forskolin-treated cells ( a ), some AQP2 is colocalized with caveolin-1 at caveolar structures in the apical membrane domain (arrows). AQP2 on the microvillous membrane is not colocalized with caveolin-1. When cells were washed and incubated without forskolin for 30 min ( b ), AQP2 was colocalized with caveolin-1 in the intracellular vesicles (arrows). Bars=100 nm.

Article Snippet: Primary antibodies were as follows: rabbit anti-AQP2 [ , ], guinea pig anti-AQP2 [ , ], goat anti-AQP2 (sc-9882; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit anti-Rab11a (Zymed, San Francisco, CA), mouse anti-EEA1 (clone 14; BD Biosciences, San Jose, CA), goat anti-EEA1 (sc-6414, Santa Cruz Biotechnology), mouse anti-caveolin-1 (clone 2234, BD Biosciences), rabbit anti-caveolin-1 (sc-894, Santa Cruz Biotechnology), mouse anti-phosphorylated caveolin-1 (clone 56, BD Biosciences), rabbit anti-cathepsin D [ ], and mouse anti-gp135 [ ].

Techniques: Incubation, Labeling